Structural Characterization of Insoluble Dextran Produced by Leuconostoc mesenteroides NRRL B-1149 in the Presence of Maltose
نویسندگان
چکیده
Rishikesh Shukla1, Shraddha Shukla1, Veselin Bivolarski2, Ilia Iliev2, Iskra Ivanova3 and Arun Goyal1* Department of Biotechnology, Indian Institute of Technology Guwahati, 781 039 Guwahati, Assam, India Department of Biochemistry and Microbiology, Plovdiv University, 24 Tzar Assen Str, BG-4000 Plovdiv, Bulgaria Department of General and Applied Microbiology, Biological Faculty, Sofia University, 8 Dragan Tzankov Str, BG-1164 Sofia, Bulgaria
منابع مشابه
Characterization of the Dextran Produced by Leuconostoc mesenteroides from Date Fruit Extract
In the present study, the production of dextran from date extract and sucrose as carbon sources by the bacterium Leuconostoc mesenteroides NRRL B512 (f) was investigated. In comparison to blue dextran (Mw≈2000 Da), dextran molecular weight was reduced (Mw2000 KDa). Flow behavior indices of dextran solutions from date extracts were investigated and rheological parameters were evaluated at concen...
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When grown in glucose or fructose medium in the absence of sucrose, Leuconostoc mesenteroides NRRL B-1299 produces two distinct extracellular dextransucrases named glucose glucosyltransferase (GGT) and fructose glucosyltransferase (FGT). The production level of GGT and FGT is 10 to 20 times lower than that of the extracellular dextransucrase sucrose glucosyltransferase (SGT) produced on sucrose...
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Leuconostoc mesenteroides NRRL B-1149 produces extracellular dextransucrase which in this study was purified using different concentrations of polyethylene glycol (PEG). The dextran produced by this enzyme is unique in that it contains α(16) and α-(13) linkages which have clinical applications. The cell free supernatant with 0.9 U/mg enzyme specific activity was subjected to fractionation by ...
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The dsrE gene from Leuconostoc mesenteroides NRRL B-1299 was shown to encode a very large protein with two potentially active catalytic domains (CD1 and CD2) separated by a glucan binding domain (GBD). From sequence analysis, DSR-E was classified in glucoside hydrolase family 70, where it is the only enzyme to have two catalytic domains. The recombinant protein DSR-E synthesizes both alpha-1,6 ...
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Different isolated strains of Leuconostoc mesenteroides produce dextran compared with that of L. mesenteroides NRRL B-512 F. Among nine newly isolated strains, strains PCSIR-4 and PCSIR-9 produced dextran of different quality. Using these two newly isolated strains, the development of dextran coincided closely with the disappearance of sucrose. The yield of dextran increased during growth and m...
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